Figure 3: Regulatory cycling of the G6pDH/NAD⁺-assembled tweezers.

Four cycles of conformational and functional transition were measured and analyzed using (a) native PAGE, (b) real-time FRET and (c) enzymatic assay. Cycles were initiated in the open state, and for each conformational change 50% excess fuel or set strands were added. All the enzyme activities were normalized to the activity of initial open tweezers.