Figure 3: Increased mass and fat droplets of BAT and impaired resistance to cold exposure in Cox7rp KO mice. | Nature Communications

Figure 3: Increased mass and fat droplets of BAT and impaired resistance to cold exposure in Cox7rp KO mice.

From: A stabilizing factor for mitochondrial respiratory supercomplex assembly regulates energy metabolism in muscle

Figure 3

(a) Appearance of brown fat pads of 4-month-old WT, KO, Tg and TgKO mice. Scale bar, 5 mm. (b) Histological section and oil red O staining of brown fats of 4-month-old WT, KO, Tg and TgKO mice. Scale bar, 100 μm. (c) Cox7rpKO mice are cold-sensitive. Body temperature of WT (blue box) and Cox7rpKO (red triangle) mice exposed to 4 °C for 5 h was measured by using a thermofocus infrared thermometer. Data are the means±s.d. of five animals per group. **P<0.01; Student’s t-test. (d) COX7RP-Tg mice demonstrate improved cold tolerance. Kaplan–Meier analysis of the body temperature was performed for mice exposed to 4 °C for 5 h, in which the end point was defined as ≤15 °C (n=7 or 8). Log-rank P-values after application of a Bonferroni correction were indicated. (e) Decreased mRNA expression of uncoupling proteins and fatty acid synthase (Fasn) in Cox7rpKO BAT. qPCR analysis was performed using total RNA from WT, KO, Tg and TgKO BAT. **P<0.01; Dunnett’s post hoc test.

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