Figure 5: HS-AFM analysis of unlabelled AQP0 on a lens cell under ambient conditions. | Nature Communications

Figure 5: HS-AFM analysis of unlabelled AQP0 on a lens cell under ambient conditions.

From: A hybrid high-speed atomic force–optical microscope for visualizing single membrane proteins on eukaryotic cells

Figure 5

(a) HS-AFM image series (960 ms frame rate) showing the typical square AQP0 lattice (a=b=6.5 nm, γ=90°) and AQP0 assembly dynamics (see arrow heads in images 3.84 and 4.80 s) neighbouring stable AQP0 molecules (arrows). A strongly protruding molecule (dashed circle) and its molecular environment have been used to analyse the displacement of the array (scale bar: 20 nm). (b) Tracking trace of the AQP0 array (the relative position of the array in the first six frames is highlighted by coloured circular markers). (c) Displacement distance histogram and (d) RMSD plot of (b) with vertical bars indicating the s.d.’s of the data points and least square linear fit determining the slope α=0.94 (red line; R2=0.97).

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