Figure 5: Coexpression of Pirt does not influence the expression of TRPM8.

The expression level of TRPM8–myc in HEK293 cells was measured by western blot after plasma membrane proteins were biotinylated and purified with streptavidin. β-Actin was used as a loading control. The expression of TRPM8 (probed with an anti-myc antibody) in whole-cell lysates (whole-cell TRPM8) or cell surface (membrane TRPM8) are at similar levels whether or not Pirt (probed with an anti-Pirt antibody) is coexpressed. The specificity of TRPM8 band was determined by the absence of the band in samples from HEK293 cells transfected with GFP alone. The molecular markers (kD) are indicated at the right side of the blots.