Figure 1: The Arabidopsis Munc13-like protein PATROL1 is required for stomatal CO2 response. | Nature Communications

Figure 1: The Arabidopsis Munc13-like protein PATROL1 is required for stomatal CO2 response.

From: A Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that is essential for stomatal responses

Figure 1

(a) Three-week-old plants were subjected to the indicated [CO2]. The subtractive images show changes in leaf temperature in response to the transfer from high to low [CO2]. (b) CO2 response of stomatal aperture in WT, patrol1 mutants and 35S:GFP–PATROL1. Three-week-old plants were incubated at the indicated [CO2]. Data are means±s.e.m. (n=60) of three independent experiments. (c) Structure of the PATROL1 gene. PATROL1 consists of 27 exons (white boxes); black boxes highlight the 5′ and 3′ untranslated regions, respectively. Arrows indicate regions used in RT-PCR (Supplementary Figs S2, S4). (d) PATROL1 encodes a protein of unknown function with a MUN domain. DUF810 (IPR008528; 194–865 aa), MUN (428–1042 aa). (e) Phylogenetic relationships based on amino-acid sequence similarities between MUN domain-containing proteins and the MUN domains of Unc13/Munc13 proteins (UNC13-MUN (Ce: C. elegans), Munc13-1 MUN (Mm: Mus musculus)), as determined with CLUSTAL X. At, Arabidopsis thaliana; Rc, Ricinus communis; Vv, Vitis vinifera; Osj, Oryza sativa subsp.japonica; Sb, Sorghum bicolor; Pt, Populus trichocarpa.

Back to article page