Figure 4: PATROL1 lines with plasma membrane-localized AHA1. | Nature Communications

Figure 4: PATROL1 lines with plasma membrane-localized AHA1.

From: A Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that is essential for stomatal responses

Figure 4

Subcellular localizations of GFP–PATROL1 and RFP–AHA1 in co-expressing transgenic plants under hypo-osmotic (a; 120 mOsM) and hyper-osmotic (b; 530 mOsM) conditions. (a,b) Enlarged images of the region enclosed in the yellow square are shown directly below. Scale bars=5 μm. (a) RFP–AHA1 in the plasma membrane was lined by PATROL1 under hypotonic condition. (b) RFP–AHA1 and GFP–PATROL1 remain at the tips of Hechtian strands in plasmolyzed guard cells. The remaining positions are indicated by arrows.

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