Figure 4: Reductively derived citrate correlates with metabolic parameters in 143B cells.
From: Reductive glutamine metabolism is a function of the α-ketoglutarate to citrate ratio in cells
(a) Reductive glutamine contribution to citrate. (b) AcCoA contribution to α-ketoglutarate. (c) Total pyruvate contribution to citrate. (d) Alterations in citrate and α-ketoglutarate levels. Correlation between the reductive glutamine contribution to citrate and (e) the AcCoA contribution to α-ketoglutarate, (f) the total pyruvate contribution to citrate, (g) the relative citrate and α-ketoglutarate levels, and (h) the α-ketoglutarate/citrate ratio. In case of lactate supplementation, M+3 in α-ketoglutarate from U-13C glutamine instead of M+2 in α-ketoglutarate from U-13C glucose was used. Ctrl denotes standard culture condition. All error bars indicate the s.d. The correlation criteria were R>0.7 or R<−0.7 and P<0.05. All P-values (Student’s t-test, two-tailed, unequal variance) and error bars are calculated from two independent replicates.
