Figure 4: Mode of action of the inhibitor NCGC00262650 is mediated through binding of AMA1. | Nature Communications

Figure 4: Mode of action of the inhibitor NCGC00262650 is mediated through binding of AMA1.

From: Disrupting malaria parasite AMA1–RON2 interaction with a small molecule prevents erythrocyte invasion

Figure 4

(a) The mode of inhibition of the small molecule was studied by a depletion assay using either his-tagged recombinant AMA1 or biotin-tagged RON2L peptide. The ability of AMA1 or RON2 to bind the inhibitor was assessed by performing invasion assays using inhibitor-depleted supernatants. Five-hundred picomoles of either recombinant AMA1 (both 3D7 and FVO allele) or RON2L peptide bound to magnetic beads was used to deplete 500 pmol of the inhibitor (final concentration 10 μM). Error bars represent±s.e.m. from two experiments. (b) Immunofluorescence assay using FITC-labelled RON2L peptide. FITC-labelled RON2 peptide binds to AMA1 in the mature schizonts in the absence of inhibitors, whereas pre-incubation with inhibitor NCGC00015280 prevents binding of the peptide. Similar results were obtained with the analogue NCGC00262650 and the inhibitor NCGC00181034 (data not shown). Scale bars, 3 μM.

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