Figure 4: Glycogenolysis blockade abolishes lipolytic signal from liver. | Nature Communications

Figure 4: Glycogenolysis blockade abolishes lipolytic signal from liver.

From: Glycogen shortage during fasting triggers liver–brain–adipose neurocircuitry to facilitate fat utilization

Figure 4

(a,b) Weight of epididymal fat pad after fasting. Effects of short hairpin RNA expression adenovirus vector targeted for Gys2 (hatched bar, a) or Pygl (filled bar, b). (n=4–8). (c) Immunoblot analysis of glycogen synthase (GS) and glycogen phosphorylase (PYGL) expression in the livers of Ad-Gys2-i or Ad-Pygl-i injected mice. (n=3–4). (d) RT–qPCR analysis of mRNA expression of glycogen synthase (Gys2) and glycogen phosphorylase (Pygl) genes. (n=3). (e) Liver glycogen content of mice infected with adenovirus expressing short hairpin RNA targeted for LacZ, Gys2 or Pygl (Ad-LacZ-i, Ad-Gys2-i or Ad-Pygl-I, respectively) (n=3–4). (f) Immunoblot analysis of Ser485-phosphorylated AMPKα (pAMPKα), total AMPKα, Ser181-phosphorylated AMPKβ (pAMPKβ) and total AMPKβ. Whole-cell lysates from the livers of mice infected with adenovirus expressing short hairpin RNA targeted for LacZ, Gys2 or Pygl (Ad-LacZ-i, Ad-Gys2-i or Ad-Pygl-i). (g) Quantified results of the data shown in (f), shown as the ratio of pAMPKα to total AMPKα and pAMPKβ to total AMPKβ. (n=3–4). All mice are analysed on 24-h fasting condition. *P<0.05 versus Ad-LacZ-i sham group (by Tukey’s post-hoc test). NS, not significant. Error bars, s.e.m.

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