Figure 2: Schematics of the PFL motif and ‘switch-OFF’ experiments following pulses of doxycycline of different duration.
From: miRNAs confer phenotypic robustness to gene networks by suppressing biological noise
(a) The tetracycline-controlled transactivator (tTA) is self-regulated, in the absence of doxycycline, by binding the tTA-responsive CMV-TET promoter, thus generating a PFL (black lines), whose dynamics is tracked by a destabilized EYFP (d2EYFP). (b) Simulated d2EYFP fluorescence of PFL cells following simulated treatment with doxycycline of different duration Δ. (c,d) Experimental d2EYFP fluorescence using the microfluidics device (solid green line) following treatment with doxycycline (red line) at time 120 min and removed after Δ=960 min (c) or 1,800 min (d); s.d. (thin green lines) is among at least three replicates; simulations (blue and orange lines) are rescaled to experimental data and also represented in b (same colours).
