Figure 1: Focal labelling of striatal and neocortical cells with GFP retroviruses in the adult rat brain.
From: Environmental impact on direct neuronal reprogramming in vivo in the adult brain
(a–c) Schematic diagrams illustrating the location of virus injection sites in parasagittal (a) and coronal (b,c) views. Boxes in a, b, and c indicate the areas shown in d, f, and j, respectively. (d–k) Distributions of GFP+ cells (dashed circles and arrowheads) in the striatum (d–h) and neocortex (i–k) at DAI-3. Sections were counterstained with methylgreen. Boxes in d, f, and j indicate the areas shown in e, g and h, j, and k, respectively. (l–w) Co-expression of neuronal markers and GFP in virus-infected cells (arrows) in the striatum (l–s) and neocortex (t–w). Dashed lines in l,o,q,r indicate the border of the matrix (M) and patch (P) compartments of the striatum. Arrows and arrowheads indicate GFP+ cells co-expressing and non-expressing, respectively, the marker shown above each panel. The lower panels m,p,s,u,w show the colocalization of relevant markers in single cells in orthogonal views of confocal z-stack images in i,o,r,t,v, respectively. Scale bar, d,e,f,i, 1 mm; e,g,h,j,k, 100 μm; l,n,o,q,r,t,v, 50 μm; m,p,s,u,w, 20 μm. Cx, neocortex; CC, corpus callosum; DG, dentate gyrus; LV, lateral ventricle; RMS, rostral migratory stream; Str, striatum; SVZ, subventricular zone.
