Figure 4: Region-specific differentiation of GFP-labelled neurons in the striatum and neocortex.

(a–g) Co-labelling of various neuronal markers and BrdU in GFP⁺ cells (arrows) in the striatum. Time points after infection, types of manipulations used and markers stained are shown above individual panels. In c–e, BrdU was administered twice each day for 3 days between DAI-0 and DAI-2. Dashed lines in a-c,f indicate the border of the matrix (M) and patch (P) compartments of the striatum. Lower panels in a–d,g show orthogonal views of confocal z-stack images of the cells indicated by arrows. Note that the overlap of green, red and blue colours in c–e is indicated as white colour. (h–y) Region-specific phenotypes of GFP-labelled neurons in the striatum (h–p) and neocortex (q–y) at DAI-28. Arrows indicate GFP⁺/NeuN⁺ neurons expressing relevant markers, whereas arrowheads indicate marker-negative GFP⁺ and/or NeuN⁺ cells. Images in n–p were obtained from control virus-infected animals, whereas all others were from Neurog2 virus-infected animals. (z,b2) Retrograde labelling of GFP⁺/NeuN⁺ cells in the striatum by FG. FG was injected into the globus pallidus ipsilateral to the virus injection site at DAI-84, and animals were analysed at DAI-91. z shows the distribution of FG fluorescence (white dots) in the striatum (the virus injection site in a dashed circle). a2 shows GFP⁺/NeuN⁺ cells co-labelled with FG detected in the area boxed in z. b2 shows confocal images (an orthogonal view in lower panels) of a neuron boxed in a2. Scale bar, a–g, 50 μm; h–y, 25 μm; z, 1 mm; a2, 50 μm; b2 and lower panels of a-d,g and b2, 20 μm.