Figure 5: Neurogenesis in the ischaemic brain.

(a–n) BrdU- and GFP-labelled neurons in the adult rat striatum (a–f) and neocortex (g–n). Circles and boxes in a,d,g,k indicate the location of virus-infected cells and the areas shown in fluorescence images, respectively. Dashed lines in b and e indicate the ventricular wall. b and e show BrdU-labelled neurons near the LV, whereas c and f show GFP-labelled neurons detected around virus-infected regions in the striatum. h and j show GFP⁺/NeuN⁺ and GFP⁺/GABA⁺ cells, respectively, in the neocortex that received control viruses, whereas l and n show GFP⁺/NeuN⁺ and GFP⁺/NeuN⁺/Glu⁺ cells, respectively, detected in Neurog2 virus-infected animals. i and m show orthogonal views of z-stack confocal images of neurons indicated by arrows in h and l, respectively. (o,p) Estimated numbers of GFP⁺/Dcx⁺ cells at DAI-3 (neocortex) and DAI-7 (striatum) (o), and GFP⁺/NeuN⁺ cells at DAI-14 (both regions) (p) under various manipulation conditions (mean±s.d., n=3–4 animals). The data regarding non-ischaemic animals are adopted from Fig. 3d,e. The numbers in parentheses show the percentage of GFP⁺/NeuN⁺ cells at DAI-14 compared with GFP⁺/Dcx⁺ cells at earlier time points. *P<0.01 compared with control viruses in Student’s t-test; $P<0.01 compared with non-ischaemic animals treated with GFs and Neurog2 in Student’s t-test. ND, not detected. Scale bar, a,d,g,k, 2 mm; b,c,e,f,h,l, 50 μm; and insets in b,c,e,f,i,j,m,n, 20 μm.