Figure 7: Differential gene expression profiles of neurosphere-forming cells derived from distinct regions of the adult mouse brain.

(a) Venn diagram showing the number of genes commonly and differentially expressed in neurospheres derived from the SVZ, neocortex and striatum. Among 3,003 genes that were selected as those that gave more than a five-fold higher hybridization signal with one or more of the adult neurosphere samples compared with the whole brain of adult mice. (b) Heat-map view of cluster analysis of 716 probe sets (637 genes) that showed more than a five-fold difference in the expression level between cortical and SVZ cells (three stripes represent three independent cultures). Neurospheres from the dorsal and ventral forebrains (dFB and vFB, respectively) of E14.5 embryos were used for comparison. (c–i) Quantitative RT-PCR analyses of the expression of TF mRNAs. The levels are normalized using glyceraldehyde-3-phosphate dehydrogenase as internal control and data are expressed as values relative to the SVZ-derived cells (designated as 1.0; mean±s.d., n=3). dFB, dorsal embryonic forebrain culture; vFB, ventral embryonic forebrain culture.