Table 1 Dwell times and export efficiencies of mRNPs.

From: High-resolution three-dimensional mapping of mRNA export through the nuclear pore

Cell type

RNA type (3.3 kb)

Label

Detecting single mRNAs on or in

Detection time per frame (ms)

Dwell time (ms)

Efficiency (%)

MEF

β-actin

YFP-MCP

NE

20

21±1

32±5

HeLa

Firefly luciferase

mCherry-MCP

NE

20

16±2

31±5

MEF

β-actin

YFP-MCP

Single NPC

2

12±1

36±5

HeLa

Firefly luciferase

mCherry-MCP

Single NPC

2

11±2

36±5

HeLa

Firefly luciferase

mCherry-MCP

Nucleus, cytoplasm and NPC-deficit NE

200

t1=620±130

t2=65,000±21,000

n/a

  1. MCP, MS2-coating protein; MEF, murine embryonic fibroblast; n/a, not applicable; NE, nuclear envelope; NPC, nuclear pore complex; YFP, yellow fluorescent protein.
  2. Intracellular dwell times and export efficiencies for single labelled β-actin and firefly luciferase mRNPs exported through single NPCs in the NE, as well as the detected two dwell times (t1 and t2) in the cytoplasm and the nucleus. The number of replicates for different types of measurements from the top to bottom in the table is 62, 75, 64, 73 and 292 respectively.
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