Table 2 Diffusion coefficients of mRNPs.

From: High-resolution three-dimensional mapping of mRNA export through the nuclear pore

Work

Cell type

RNA type

Nucleus (μm2 s−1)

NPC (μm2 s−1)

Cytoplasm (μm2 s−1)

Detection speed (ms per frame)

    

N

S

C

  

7

U2OS

Human (2.3 kb)

0.009

n/a

n/a

n/a

0.011

1000

31

U2OS

Human (3.4 kb)

0.04

n/a

n/a

n/a

n/a

375

32

CHO

Hamster (n/a)

0.033 (86%) 0.0006 (14%)

n/a

n/a

n/a

0.029

300

This paper

HeLa

Firefly luciferase (3.3 kb)

0.067 (47%) 0.009 (53%)

n/a

n/a

n/a

0.49 (81%) 0.05 (19%)

100

3, 6

MEF

β-actin (3.3 kb)

1.3

n/a

0.06

n/a

n/a

20

This paper

HeLa

Firefly luciferase (3.3 kb)

3.42 (23%) 0.61 (67%) 0.09 (7%) 0.03 (3%)

0.16

0.07

0.19

2.24 (57%) 0.37 (38%) 0.05 (5%)

2

  1. C, cytoplasmic side of the NPC; CHO, Chinese-hamster ovary; MEF, murine embryonic fibroblast; N, nucleoplasmic side of the NPC; n/a, not applicable; S, central scaffold region of the NPC.
  2. Diffusion coefficients of mRNPs in nucleus, NPC and cytoplasm. Where applicable, the fraction of total detected mRNPs with the indicated diffusion coefficient is given in parentheses.
Back to article page