Figure 6: Validation of regulons.
From: Master regulators of FGFR2 signalling and breast cancer risk
(a) Enrichment of known binding motifs for ESR1, FOXA1 and GATA3 in each of their inferred regulons. The occurrence of motif sites is shown as the distance between the TSS of the genes in each regulon and the nearest motif encountered (red line). This was compared with the occurrence of random sites of the same length in the same regulons derived for a random motif (black line; mean±s.d.). Motifs are taken from Transfac. (b) Enrichment of binding sites of the ESR1, FOXA1, GATA3 and SPDEF regulons in SPDEF ChIP-seq data obtained in MCF-7 cells. A background distribution is shown as a reference line (grey line) and represents the distance between the TSS and a random peak placed in the same chromosome. (c–f) ERα occupancy changes after FGF10 signalling (mean±s.e.m.) in three technical repeats. ERα binding was analysed by ChIP–RT–PCR at the regulatory regions of four genes: (c) MYC, (d) GREB, (e) EGR2 and (f) the breast cancer susceptibility gene TOX3. Enrichment is shown relative to a negative control from the CCND1 locus. Cell stimulation with E2, FGF10 and PD (PD173074) is indicated in each panel.
