Figure 3: No increase in the replication ability in the control TcRR cycles without compartmentalization.
From: Darwinian evolution in a translation-coupled RNA replication system within a cell-like compartment
Two independent serial TcRR cycles were performed using the method represented in Fig. 2a, without compartmentalization (Bulk); in these cycles, the initial plus-strand concentration was 1 nM, corresponding to the effective plus-strand concentration in the experiment with compartmentalization. The figure shows the average minus-strand RNA concentration after RNA replication. In both experiments, the minus-strand RNA gradually decreased and was no longer recovered after round 17. For comparison, the result of a TcRR cycle with compartmentalization (the same experiment represented in Fig. 2) is also plotted (Compartment).
