Figure 2: Inactivation of ABP1 does not affect the expression of TIR1 and AFBs.
From: Auxin-Binding Protein 1 is a negative regulator of the SCFTIR1/AFB pathway
(a) Quantitative RT–PCR analysis of TIR1, AFB1, AFB2 et AFB3 in WT (in white) and SS12K9 (in grey) after various times of ethanol induction. All data were normalized with respect to ACTIN2-8 and expressed in equivalent ACTIN units. Error bars are s.d., n=6 (three biological replicates, each in two experimental repeats). (b) Immunodetection of TIR-HA reporter protein in Col0 and SS12K9 backgrounds, with or without inactivation of ABP1. Red ponceau staining is included as a loading control. Full image of blots are shown as a Supplementary Fig. S1.
