Figure 6: An ion-pair interaction between K195 and D579 is important for NAD+ signalling.
From: Molecular determinants of common gating of a ClC chloride channel
(a) Protopore and (b) common gating curves for K195 and D579 mutant channels (open symbols). Also shown in panel (b) are common gating curves in the presence of 3 mM intracellular NAD+ (filled symbols). Data are means and s.e.m., and solid lines represent fits of equation (1) to experimental data points. (c) Mean ΔV 1/2common of K195 and D579 mutant channels by 3 mM intracellular NAD+. Data are means and linearly propagated values of s.e.m. For measurements with 3 mM NAD+ n=5 (WT ClC-1), 5 (K195D), 3 (D579K) and 3 (K195D/D579K) separate experiments. Asterisks denote statistical significance determined by one-way ANOVA (P<0.0001). n=3 (K195D, K195D/D579K, D579K (3NAD+), K195D/D579K (3NAD+), n=4 (WT ClC-1, D579K) or n=5 (WT (3NAD+), K195D (3NAD+)). Parameters of the fits of equation (1) to experimental data points for this figure are listed in Supplementary Table S3.
