Table 1 A bifurcated salt bridge linking helices G-I is important for gating.
From: Molecular determinants of common gating of a ClC chloride channel
n | V 1/2 (mV) | z (e0) | P min | V1/2A+(V1/2B-V1/2WT) (mV) | |
|---|---|---|---|---|---|
Common gating | |||||
WT ClC-1 | 4 | −51.6±1.7 | 1.05±0.04 | 0.21±0.02 | — |
R300D | 3 | −57.6±1.8 | 0.83±0.02 | 0.20±0.02 | — |
R304D | 4 | −61.7±2.2 | 1.22±0.05 | 0.38±0.01 | — |
D265R | 3 | +30.0±1.1 | 0.82±0.02 | 0.17±0.01 | — |
D265R R300D | 3 | −39.9±1.2 | 1.17±0.01 | 0.25±0.01 | +24.0±2.7(<0.0001) |
D265R R304D | 5 | −44.9±1.1 | 1.15±0.03 | 0.20±0.01 | +19.9±2.1(<0.0001) |
R300D R304D | 3 | −14.3±2.1 | 0.59±0.02 | 0.14±0.02 | −67.7±3.3(0.0001) |
D265R R300D R304D | 4 | −48.1±1.2 | 1.08±0.02 | 0.24±0.01 | +67.3±3.0(<0.0001) |
Protopore gating | |||||
WT ClC-1 | 4 | −81.6±1.2 | 1.26±0.04 | 0.17±0.02 | — |
R300D | 3 | −94.4±2.6 | 1.22±0.21 | 0.23±0.03 | — |
R304D | 4 | −81.5±1.7 | 1.00±0.08 | 0.10±0.02 | — |
D265R | 3 | −30.1±3.7 | 0.77±0.14 | 0.36±0.02 | — |
D265R R300D | 3 | −72.4±1.2 | 1.32±0.01 | 0.15±0.01 | −42.9±4.7(0.0042) |
R300D R304D | 3 | −80.6±3.3 | 0.93±0.07 | 0.06±0.04 | −93.4±3.3(0.0509) |
D265R R304D | 5 | −81.7±3.3 | 1.01±0.08 | 0.15±0.04 | −30.0±4.4(<0.0001) |
D265R R300D R304D | 4 | −72.0±1.2 | 1.26±0.05 | 0.17±0.01 | −29.1±3.7(<0.0001) |
