Figure 3: Elevated temperatures inhibit ETI responses.
From: Plant immune response to pathogens differs with changing temperatures
(a) Activation of WRKY46 by AvrRpt2 at different temperatures. Four-week-old Dex-AvrRpt2 plants were hand-inoculated with H2O or 10 μM Dex, or the protoplasts were transfected with AvrRpt2 or a vector control, and incubated at different temperatures for 6 h before sample collection for RNA isolation. The gene activation fold is presented as the ratio of AvrRpt2 expression to controls with the mean±s.e.m. (n=3) from three independent biological replicates. (b) Activation of WRKY46 by AvrRpm1 or AvrB at different temperatures. The protoplasts were transfected with AvrRpm1, AvrB or a vector control and incubated at different temperatures for 6 h before sample collection for RNA isolation. The data are shown as mean±s.e.m. (n=3) from three independent biological replicates. * indicates a significant difference with P<0.05 analysed with the SPSS software one-way ANOVA analysis when compared with corresponding data from 16 °C. (c) Cell death in DEX-avrRpt2 transgenic plants at different temperatures. The DEX-avrRpt2 transgenic plants were hand-inoculated with H2O or 10 μM Dex and incubated at different temperatures. The cell death was recorded 24 hpi for plants incubated at 16, 23, 28 and 32 °C, 40 hpi for plants at 10 °C and 48 hpi for plants at 4 °C. The cell death was shown with Trypan blue staining and % indicates the percentage of wilting leaves of total inoculated leaves. The expression of avrRpt2 after DEX treatment is shown. Actin is the control for RT-PCR. The above experiments were repeated three times with similar results.
