Figure 3: Adhesion profile and migration of SCC-2 cells after silencing or overexpression of CTNNA2 and CTNNA3.

(a) Following CTNNA2 and CTNNA3 silencing, binding capacity of SCC-2 on different extracellular matrix components was assessed. Affinity is expressed as relative fluorescence units (RFU). ‘i’ refers to CTNNA2 or CTNNA3 silenced cells, and ‘o’ to cells overexpressing CTNNA2 or CTNNA3. Col I, type-I collagen; Col II, type-II collagen; FN, fibronectin; LN, laminin. Statistical analyses were performed by Student’s t-test with *P<0.05 and **P<0.01. Error bars represent mean±s.e.m. of three biological replicates. (b) Distance covered by the migration edge from the initial site was measured at different times and graphically represented. Pictures at different times from a representative assay are shown. Statistical analyses were performed by Student’s t-test with *P<0.05 and **P<0.01. Error bars represent mean±s.e.m. of three to five biological replicates. Scale bars, 250 μm.