Figure 4: Cellular features and β-catenin transcriptional activity of SCC-40 cells after silencing of CTNNA2 and CTNNA3. | Nature Communications

Figure 4: Cellular features and β-catenin transcriptional activity of SCC-40 cells after silencing of CTNNA2 and CTNNA3.

From: Cell–cell adhesion genes CTNNA2 and CTNNA3 are tumour suppressors frequently mutated in laryngeal carcinomas

Figure 4

(a) Following CTNNA2 and CTNNA3 silencing (top), binding capacity of SCC-40 on different extracellular matrix components was assessed (bottom). Col I, type-I collagen; Col II, type-II collagen; FN, fibronectin; LN, laminin. Affinity is expressed as relative fluorescence units (RFU). (b) Distance covered by the migration edge from the initial site was measured at different times and graphically represented. Migration pictures at different times from a representative experiment are shown. (c) CTNNA2 and CTNNA3 silencing increased invasive capacity of SCC-40 cells. Number of invasive cells were counted and graphically represented. (d) β-catenin transcriptional activity in SCC-40 cells following CTNNA2 and CTNNA3 interference. TOP, reporter with optimal Tcf-binding site; FOP, background assessed with Far-from-optimal Tcf-binding site. Statistical analyses were performed by Student’s t-test with *P<0.05 and **P<0.01. Error bars represent mean±s.e.m. of three biological replicates. Scale bars, 250 μm and 50 μm in b and c, respectively.

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