Figure 7: In vivo functionality of GrlR–GrlAΔ asymmetric complex.
From: Structure of GrlR–GrlA complex that prevents GrlA activation of virulence genes
(a) Immunoblot analysis using anti-GFP antibodies to compare the expression of GFP via the ler promoter. The used strains include a deletion mutant of grlRA (control; indicated as GrlR−A−); this mutant complemented with plasmids expressing GrlA, GrlAΔ, GrlRA and GlRAΔ, as indicted above the lanes. All strains contained a compatible GFP-expressing plasmid via the ler promoter. (b) Secreted proteins were concentrated from supernatants of bacterial culture grown in DMEM and resolved using 12% SDS–PAGE. These samples were then transferred to a PVDF membrane and analysed using a monoclonal antibody against representative secretory protein EspB. The strains used are as in a, but all lack the GFP-expressing plasmid. Strains are indicated above the lanes as in a.
