Figure 8: In vivo analysis of the importance of key HTH motif residues in GrlA–DNA binding.
From: Structure of GrlR–GrlA complex that prevents GrlA activation of virulence genes
(a) Secreted proteins were concentrated from supernatants of bacterial culture grown in DMEM and resolved in 12% SDS–PAGE. The samples were then transferred to a PVDF membrane and analysed using polyclonal antiserum against representative secretory protein EspB. The used strains include a deletion mutant of grlRA (control) (indicated as GrlR−A−); this mutant complemented plasmids expressing GrlA or different GrlAΔ substitutions, as indicated above the lanes (b). Immunoblot analysis using anti-GFP antibodies to compare the expression of GFP via the ler promoter. The used strains are similar to those described in a, with the exception that all contain a compatible GFP expression plasmid via the ler promoter. Strains are indicated above the lanes.
