Figure 1: Characterization of the muse5-1 mos4 snc1 triple mutant.
From: Mitochondrial AtPAM16 is required for plant survival and the negative regulation of plant immunity
(a) Morphology of 3-week-old soil-grown plants of wild-type (WT), snc1, mos4 snc1 and muse5-1 mos4 snc1. Scale bar represents 1cm. (b) PR-1 and PR-2 expression in WT, snc1, mos4 snc1 and muse5-1 mos4 snc1 as determined by RT–PCR. Total RNA was extracted from 2-week-old plants grown on 1/2 MS medium and reverse transcribed to cDNA. PR-1, PR-2 and Actin-1 were amplified by 28 cycles of PCR using equal amounts of total cDNA, and the products were analysed by agarose gel electrophoresis followed by ethidium bromide staining. (c) GUS staining of 2-week-old plate-grown seedlings of WT, snc1, mos4 snc1 and muse5-1 mos4 snc1. All plants carry the pPR2::GUS reporter gene. (d) Quantification of H.a. Noco2 sporulation on the indicated genotypes. Two-week-old plants were inoculated with H.a. Noco2 at a concentration of 105 spores per ml of water. The spores were quantified with a hemocytometer 7 days after infection. Bars represent means±s.d. (n=4 with five plants each). Similar results were obtained in three independent experiments.
