Figure 2: Positional cloning of muse5-1.
From: Mitochondrial AtPAM16 is required for plant survival and the negative regulation of plant immunity
(a) Map position of muse5-1 on chromosome 3. BAC clones are indicated. (b) Gene structure of MUSE5 (At3g59280). Boxes indicate exons and lines indicate introns. Grey regions show the UTRs. The two arrows indicate the start and stop codon, respectively. The asterisk indicates where the G-to-A mutation in muse5-1 occurred. (c) cDNA sequence comparison between MUSE5 and muse5-1. In muse5-1, one nucleotide of the third exon was spliced out due to the G-to-A mutation at the intron–exon splice junction that creates a new 3′ splice site, causing a reading frame shift. (d) Phylogenetic relationship between PAM16 and its orthologs. The orthologs are represented by the species names. Phylogenetic analysis was carried out using software MEGA v5.05. All amino acid sequences were compiled in FASTA format and used to generate alignment first. Maximum likelihood (ML) tree was implemented assessing with 1,000 bootstrap replications. (e) Full-length amino acid sequence alignments of MUSE5, MUSE5L and PAM16 proteins from S. cerevisiae (Sc) and S. pombe (Sp). The conserved PAM16 domain and degenerate J domain (J-like domain) are underlined by solid and dotted lines, respectively. Black boxes indicate regions in which at least two of three residues are identical and grey boxes indicate conserved residues.
