Figure 5: Inhibition or deletion of Cx40 or Cx37 does not affect TXB₂ synthesis in platelets.

The levels of TXB₂ were measured by immunoassay in human PRP following stimulation with 1 μg ml⁻¹ CRP-XL in the presence or absence of 100 μg ml⁻¹ of ⁴⁰Gap27 or ^37,43Gap27 (a). Similarly, TXB₂ levels were measured in Cx37^−/− (b) and Cx40^−/− (c) mouse platelets upon stimulation with 1 μg ml⁻¹ CRP-XL. The levels of TXB₂ obtained in human platelets were shown as concentrations. The levels of TXB₂ obtained with resting platelets in Cx37^+/+ or Cx40^+/+ mouse platelets were taken as 100% for comparison with Cx37- or Cx40-deficient mouse platelets. R, resting platelets. Data represent mean±s.d. (n=3; Student’s t-test was used for statistical analysis).