Figure 8: Cx40 regulates clot retraction and haemostasis.
The effect of 40Gap27 on clot retraction using human PRP was analysed in vitro for 90 min (a,b). The effect of Cx40 deficiency on clot retraction was analysed (c,d). Data represent mean±s.d. (n=4; Student’s t-test, *P<0.05 and **P<0.01). Deletion of Cx40 or the effect of 40Gap27 (estimated plasma concentration: 100 μg ml−1) on haemostasis in mice was analysed by measuring the bleeding time after tail tip excision. The bleeding time obtained with Cx40−/− mice were compared with Cx40+/+ mice (e). Similarly, the bleeding time obtained with scrambled peptide- (estimated 100 μg ml-1) treated group was compared with 40Gap27-treated mice (f). Data represent mean±s.d. (n=5 for Cx40+/+or Cx40−/− mice and n=5 mice for scrambled peptide control or 40Gap27 treated). The significance between control and treated groups, and P-values (as shown) were calculated by non-parametric Mann–Whitney test using GraphPad Prism.
