Figure 11: Loss of Pellino3 in mice leads to increased hepatoxicity and lethality in response to TNF in vivo.
From: Pellino3 targets RIP1 and regulates the pro-apoptotic effects of TNF-α
Age- and sex-matched Pellino3+/+ and Pellino3−/− mice were injected intraperitoneally with TNF and D-galactosamine (n=11) or D-galactosamine alone (n=5). (a) Survival was monitored every hour for 36 h. (b) Liver tissues were collected and photographed at 12 h after injection. Scale bar, 3 mm. (c) Hepatic tissue was collected 12 h after injection, sectioned and subjected to histological analysis using H&E staining and immunohistochemistry using anti-cleaved caspase 3 antibody. The white arrows indicate hepatocytes with fragmented nuclei. Scale bar, 50 μm. (d) Cell lysates were generated from hepatic tissue 12 h post injection and probed by immunoblotting for levels of cleaved caspase 3, caspase 8 and β-actin. (e) Blood samples were taken from mice 7 h after injection and serum assayed by ELISA for levels of IL-6, IL-10 and TNF. Data represent the mean +/− s.e.m. of four independent experiments. Full-length images of immunoblots are shown in Supplementary Fig. S18.
