Table 2 A3Fc-CD2 ssDNA-binding site mutations.
From: Structural determinants of HIV-1 Vif susceptibility and DNA binding in APOBEC3F
A3Fc-CD2 variant | Expression level | K d (μM) | T m (°C) |
|---|---|---|---|
WT | +++++ | 7.2 | 60 |
W277A | ++ | n.d. | 57 |
R305A | + | n.d. | − |
Y307A | +++++ | 410 | 58 |
Y308A | +++++ | 18 | 58 |
F309A | +++++ | 31 | 55 |
W310A | ++++ | 27 | 55 |
D313A | +++++ | 220 | 58 |
Y314A | +++++ | 68 | 52 |
K367A | +++++ | n.d. | 56 |
Q369A | +++ | 760 | 58 |
