Table 3 A3Fc-CD2 Vif-binding site mutations.
From: Structural determinants of HIV-1 Vif susceptibility and DNA binding in APOBEC3F
A3Fc-CD2 variant | Expression level | K d (μM) | T m (° C) |
|---|---|---|---|
Hydrophobic Vif binding site | |||
 WT | +++++ | 1.1 | 60 |
 L255A | + | n.b. | Unstable |
 L255D | ++ | n.b. | 62 |
 F258A | + | n.b. | Unstable |
 C259K | ++ | n.b. | 62 |
 C259S | +++++ | n.b. | 54 |
 I262A | + | n.b. | 62 |
 L263A | + | 1.1 | Unstable |
 S264D | +++++ | 1.3 | 54 |
 Y269A | + | n.b. | 50 |
 F290A | + | n.b. | 53 |
 F290K | + | n.b. | 62 |
 H294A | +++ | n.b. | 55 |
 H294D | ++ | n.b. | 60 |
Negatively charged Vif binding site | |||
 E286A | +++++ | n.b. | 56 |
 E289Q | +++++ | n.b. | 56 |
 E316Q | +++++ | n.b. | 56 |
 R319A | +++++ | 0.5 | 50 |
 S320A | +++++ | n.b. | 53 |
 E324Q | ++++ | n.b. | 57 |
