Figure 4: Wnt pathway components at the receptor level are required for canonical activation in colon cancer cells.
From: Wnt secretion is required to maintain high levels of Wnt activity in colon cancer cells
(a) Knockdown of Lrp6, Dvl1 or Dvl3 reduces Wnt reporter activity in HCT116 and DLD1 cells. Cells stably transfected with a TCF4/Wnt reporter were reverse transfected with the indicated siRNAs and assessed for luciferase activity 72 h later. (b) Silencing of Dvl1 and Dvl3 reduces Wnt target gene expression in DLD1 cells. DLD1 cells were reverse transfected with the indicated siRNA and assessed for the expression of the Wnt target gene (AXIN2), as well as DVL1 and DVL3, by quantitative PCR 72 h later. (a,b) Data from four to five independent experiments are shown as mean±s.e.m.
