Figure 3: Identification of the triple emission bands.
From: Probing water micro-solvation in proteins by water catalysed proton-transfer tautomerism
(a,b) Absorption (black) and emission spectra (red) of (2,7-aza)Trp are shown in a and 2,7-diazaindole are shown in (b) in neutral water. Further, the decomposed PL spectra are shown (violet, blue and green triangles). λex=290 nm. (c) Absorption and emission spectra of N(1)-Me and N(2)-Me in neutral water. (d) The emission decay dynamics of (2,7-aza)Trp monitored at 320 nm (violet) and 540 nm (green). (e) The emission decay dynamics of (2,7-aza)Trp monitored at 380 nm. (f) The emission decay dynamics of N(1)-Me and N(2)-Me. The instrument response and the fitting curves are marked in black and red lines, respectively. The residuals of the fit of emission decay dynamics as shown in Supplementary Fig. S8.
