Figure 2: Regulation of GSK3b activity and dendritic growth by neurotrophic factors.
From: Activity-dependent regulation of dendritic growth and maintenance by glycogen synthase kinase 3β
(a) Bar graph summarizes the effects of 2-day bath application of K252a (200 nM), K252b (200 nM) or BDNF (100 ng ml−1) on the pS9/Total-GSK3β ratio in the somatodendritic region. Numbers indicate the number of cells examined. (b) Representative confocal images of hippocampal neurons expressing EGFP treated with K252a, at 1 and 5 days post transfection. Arrows indicate shrinking dendritic branches. Scale bar, 50 μm. (c,d) Bar graphs show the normalized changes (+5 versus+1 d or +6 versus+2 d) in total dendritic length and branch number under different conditions. Numbers indicate the total number of cells examined (from at least three independent batches of culture). Error bars: s.d. *P<0.05 comparing with the EGFP K252b treated group, #P<0.05 comparing between the labelled groups (Student’s t-test).
