Figure 4: Reduction in dendritic branches by GSK3b activation in organotypic hippocampal slices.
From: Activity-dependent regulation of dendritic growth and maintenance by glycogen synthase kinase 3β
(a) Representative two-photon confocal images of CA3 hippocampal neurons expressing EGFP alone, EGFP with GSK3β-S9A (S9A) and EGFP with GID5–6 (GID5–6) at 2 and 10 days after biolistic transfection. Images were inverted in grayscale for presentation. Composite tracings of the apical dendrites are shown on the right, with the red and green segments representing shrinkage and growth, respectively. Scale bar, 100 μm. (b) Bar graphs show changes in total dendritic length and branch number as labelled. For each condition, data are normalized to the 2 day after transfection values. Numbers in brackets indicate the number of cells examined (from at least three independent batches of culture). Error bars: s.d. *P<0.05 and **P<0.005 by Student’s t-test.
