Figure 6: Reduction of inhibitory synaptic strength by GSK3b activation.
From: Activity-dependent regulation of dendritic growth and maintenance by glycogen synthase kinase 3β
Whole-cell patch-clamp recordings showing the changes in postsynaptic currents in hippocampal neurons expressing EGFP, EGFP with wild-type GSK3β (WT), EGFP with GSK3β-S9A (S9A), and EGFP with GID5–6. Cells were transfected at DIV13 and recordings were performed between DIV17-19. (a) Measurements of the miniature excitatory and inhibitory postsynaptic currents. Sample traces of mEPSC and mIPSC are shown on the top and the quantified frequencies and amplitudes are shown in the bar graphs below. mIPSCs are inward at −80 mV because ECl=0 mV. *P<0.05 comparing with the EGFP group (Student’s t-test). (b) Bar graphs show the spontaneous postsynaptic currents (sPSCs) in neurons expressing GSK3β-S9A and normalized to neighbouring, non-transfected neurons (Ctrl). *P<0.05 comparing with the control group (Student’s t-test). Numbers indicate the total number of cells examined for each group (from at least three independent batches of culture). Error bars: s.e.m.
