Figure 3: Evaluation of assembly integrity.
From: High genome heterozygosity and endemic genetic recombination in the wheat stripe rust fungus
Scaffold 475 of the Pst-CY32 assembly has only one PE supported gap, and it matches to one region of fosmid clone_Txjkx that was fully sequenced by Sanger sequencing. The same region consists of 11 contigs in the Pst-130 assembly generated solely by WGS Illumina sequencing. Therefore, the fosmid-to-fosmid strategy greatly improved assembly integrity. High PE (paired end) support density indicates high assembly confidence. PE supported gaps are regions that may have SVs derived from genome heterozygosity. Green, yellow and orange curves show paired-end supports from sequences of 6-, 2- and <1-kb insert libraries, respectively.
