Figure 5: Loss of MutS induces ATF-2 expression and attenuates nucleolar stress phenotypes. | Nature Communications

Figure 5: Loss of MutS induces ATF-2 expression and attenuates nucleolar stress phenotypes.

From: Base excision repair AP endonucleases and mismatch repair act together to induce checkpoint-mediated autophagy

Figure 5

(a) Expression of ATF-2::GFP in 5-FU-treated hermaphrodites fed control or RNAi targeting the indicated genes (scale bars, 20 μm). (b) The number of animals harbouring embryos with excessive ATF-2::GFP expression was scored in at least 150 animals and given as the fraction (%) of the total. (c) N2 or msh-6(pk2504) embryos were continuously monitored under differential interference contrast microscopy (scale bars, 5 μm) from the three-cell stage until completion of the four-cell stage. Magnified images of representative nuclei are shown. (d) The fraction of embryos with visible, enlarged nucleoli (arrows) after 5-FU treatment was scored. Bar graphs represent mean±s.d. from three independent experiments.

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