Figure 1: Corticosteroids attenuate IL-33-induced airway inflammation and NH-cell accumulation. | Nature Communications

Figure 1: Corticosteroids attenuate IL-33-induced airway inflammation and NH-cell accumulation.

From: Thymic stromal lymphopoietin induces corticosteroid resistance in natural helper cells during airway inflammation

Figure 1

(a) Airway inflammation was induced with administration for 3 consecutive days of IL-33 (1 μg per animal, intranasal) in C57BL/6 mice. Dexamethasone (Dex, 5 mg kg−1) was injected intraperitoneally at 24 and 1 h before IL-33 administration for some mice. (b,c) Number of eosinophils in the BALF (b) and NH cells in the lungs (c) 4 days after the final administration of IL-33 or vehicle (PBS). Lung NH cells were counted as Lin− IL-7R+CD25+c-Kit+Sca-1+ST2dimcells. Mean±s.e.m., n=3 for each group. *P<0.05 (Student’s t-test) (d) Flow cytometric analysis of NH cells in the lung. (e) Intracellular staining of IL-5 and IL-13 in CD4+ T cells and NH cells. (f,g) Amounts of IL-5 (f) and IL-13 (g) in the BALF were measured by ELISA. Mean±s.e.m., n=3 for each group. *P<0.05 (Student’s t-test). (h) Histology of airways from these mice stained with hematoxylin and eosin (left panels) or periodic acid–Schiff (PAS)-alcian blue (right panels). Scale bar, 100 μm. Data are representatives of three independent experiments.

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