Figure 4: An anti-TSLP antibody ameliorates corticosteroid resistance in an OVA plus IL-33-induced asthma model. | Nature Communications

Figure 4: An anti-TSLP antibody ameliorates corticosteroid resistance in an OVA plus IL-33-induced asthma model.

From: Thymic stromal lymphopoietin induces corticosteroid resistance in natural helper cells during airway inflammation

Figure 4

(a) OVA, IL-33 and dexamethasone (5 mg kg−1) were administered to OVA-sensitized mice in combination with an anti-TSLP antibody (25 μg) or an isotype control antibody. (b,c) Number of eosinophils in the BALF (b) and NH cells in the lungs (c) 4 days after the final administration of OVA/IL-33/Dex/antibodies. Mean±s.e.m., n=4 for each group. *P<0.05 (Student’s t-test). (d,e) Concentrations of IL-5 (d) and IL-13 (e) in the BALF were measured by ELISA. Mean±s.e.m., n=4 for each group. *P<0.05 (Student’s t-test). (f) Representative histology of airways stained with hematoxylin and eosin (left panels) or periodic acid–Schiff (PAS)-alcian blue (right panels). Scale bar, 200 μm. (g,h) Number of eosinophils in the BALF (g) and NH cells in the lung (h) in wild-type (WT) and Tslpr−/− mice sensitized with and exposed to OVA with or without IL-33 (0.1 μg) and/or dexamethasone (5 mg kg−1). Mean±s.e.m., n=3–6 for each group. NS, not significant. *P<0.05 (Student’s t-test). Data are representatives of three independent experiments.

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