Figure 6: Syndecan-3 and glypican-1 are the target HSPGs of SCI-induced HS upregulation in T1KO mice.
From: Chondroitin sulphate N-acetylgalactosaminyl-transferase-1 inhibits recovery from neural injury
(a,b) Syndecan-3 and glypican-1, HSPGs derived from neurons, were expressed in T1KO mice after SCI (2 weeks). (a) Dot-blot analysis of the major core proteins decorated with CSPG or HSPG. (b) Immunohistochemistry of syndecan-3 and glypican-1 after SCI (2 weeks) in WT and in T1KO mice. Scale bar, 1 mm. (c) HSase-treated HSPGs detected by 3G10, the antibody that recognized the HS stub. At least three distinct HSPGs were recognized by 3G10 in T1KO SCI mice, but not in WT SCI mice, including 180 kDa (syndecan-3) and 65–80 kDa (glypican-1). (d) Western-blot analysis of PGs near the lesion sites before (−) and after (+) HSase treatment (5 mU; 37 °C, 3 h). Notably, syndecan-3 and glypican-1 were the main PGs with HSase-sensitive HS in injured T1KO mice. The data for CSPG core proteins (aggrecan, NG2 or phosphacan) are shown only for the injured T1KO mice, and each CSPG was HSase insensitive. Arrows indicate the position of deglycosylated proteins. Numbers on the right indicate molecular masses (kDa).
