Figure 3: DYF-19 regulates the ciliary entry of assembled IFT particles. | Nature Communications

Figure 3: DYF-19 regulates the ciliary entry of assembled IFT particles.

From: Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes

Figure 3

(a) At the base of Wt or dyf-19 cilia, the IFT-A component CHE-11 and the IFT-B component OSM-6 co-localize, presumably below the TZ. (b) In BiFC analyses, fluorescence complementation of CHE-11::VN-IFT-20::VC, DYF-2::VN-VC::BBS-9 and DYF-2::VN-VC::BBS-9 occurs along the entire cilium in Wt, but only at the ciliary base in dyf-19 mutants. Reconstituted CHE-11::VN-IFT-20::VC (c) and DYF-2::VN-VC::BBS-9 (d) localize just below the TZ marker in dyf-19 mutants. VN: N terminus of Venus yellow fluorescent protein (YFP); VC: C terminus of Venus YFP. Bars, 5 μm.

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