Figure 3: Strong accumulation of H2a and calnexin in the ERQC in the presence of visible Htt96Q aggregates in striatal neurons.

(a) The localization of H2a accumulation is not correlated with Htt96Q aggregate localization. Similar to the experiment in Fig. 1c. (b,c) Non-striatal cells are less affected by large Htt96Q aggregates. Similar to the experiments in Fig. 1c,d, but with NIH 3T3 and N2a cells. (d) As in a, but with BiP-GFP expression and Htt96Q-Cherry. Htt96Q aggregation does not affect the disperse pattern of BiP-GFP through the ER. Scale bar, 10 μm in all panels.