Figure 7: Progressive ERAD substrate accumulation in the ERQC before appearance of visible Htt aggregates. | Nature Communications

Figure 7: Progressive ERAD substrate accumulation in the ERQC before appearance of visible Htt aggregates.

From: Soluble forms of polyQ-expanded huntingtin rather than large aggregates cause endoplasmic reticulum stress

Figure 7

(a) The drop in Htt synthesis using TEToff regulation was verified by metabolic labelling for 30 min with [35S]-(Met+Cys) and IP of Htt96Q-Cherry expressed in STHdhQ7/7 cells following incubation with Dox for the indicated periods. (b) Live imaging of a representative STHdhQ7/7 cell expressing Htt96Q-Cherry and H2a-GFP, after shut-off of Htt96Q-Cherry synthesis. Arrow: appearance of a visible large Htt96Q-Cherry aggregate. Arrowhead: H2a-GFP juxtanuclear accumulation. Scale bar, 10 μm. The corresponding movie can be seen in Supplementary Movie 1. (c) H2a-GFP accumulation in the ERQC relative to Htt96Q-Cherry aggregation (from seven independent experiments). Zero: first appearance of a visible Htt96Q-Cherry aggregate. Each line colour represents the same individual cell in both graphs. (d) As in c, but in cells with the presence of visible Htt96Q-Cherry aggregates and H2a-GFP accumulation from the beginning of the observation, (from six independent experiments).

Back to article page