Figure 6: PTB1 affects rice PSSR by controlling PT growth.
From: Natural variation in PTB1 regulates rice seed setting rate by controlling pollen tube growth
(a) Panicle phenotype of each category, as defined by PSSR (lower category, PSSR⩽30%; moderate category, 30%⩽PSSR⩽70%; higher category, PSSR⩾70%). (b) PT growth in each category (from left to right, lower, moderate and higher categories). Arrows indicate the chalazal end of the embryo sac and the micropyle. (c) The correlation between PSSR and the expression level of introduced PTB1. Data are given as mean±s.e.m. (n=3). PSSRs are listed in Supplementary Table S3. (d) PT growth abnormalities observed in several lines with lower PSSR. The region boxed using a white line is shown magnified in another window in the same panel. Arrows indicate the chalazal end of the embryo sac and the micropyle (in WT) and the PT tip (in 6685, 7302R and C1352). (e) Determinants of natural PSSR variations. PSSR and pollen fertility are presented as the mean±s.e.m. (n=3), and the normal pistil ratio was calculated by comparing the ratio of the PT-normal-grown pistils to the total of individuals observed using fluorescence microscopy (n>30). rpp, the correlation between PSSR and pollen fertility; rpn, the correlation between PSSR and normal pistil ratio. (f) The correlation between natural PSSR and the expression level of native PTB1 in a natural population. All r values are based on two-tailed Pearson correlation analyses. Samples for fluorescence microscopic observation were collected at 2 h AP, n>30. Scale bars, 5 cm in (a) and 400 μm in (b) and (d).
