Figure 3: Insights into the mechanisms of electron transfer from transcriptomic data.

(a) Fold changes observed for the differentially expressed genes in the hyb K.O. strain and CS K.O. strain. (b) Metabolic interpretation of the transcriptomic differences in the hyb K.O. strain relative to the WT strain. The main genes unregulated in the hyb K.O. strain include the omcS that are associated with the pathway for DIET in the consortium. (c) Metabolic adjustments in the CS K.O. strain based on the transcriptomic data. The elimination of interspecies electron transfer via acetate oxidation confers a suboptimal direct electron transfer phenotype to the consortium. This is mirrored by the significant suppression of the electron transfer pathway involved in DIET in G. metallireducens. All flux values are in mmol per gram DW per h.