Figure 3: Dicer and cyclin D1 govern heterochromatic H3K9 trimethylation.
From: Cyclin D1 induction of Dicer governs microRNA processing and expression in breast cancer
(a) HCT116 cells were stained with an antibody specific for trimethyl-H3K9 and DAP1 strained for nuclei. H3K9 trimethylation was assessed in Dicer+/+ versus Dicer−/− HCT116 cells treated with cyclin D1 siRNA or control. Quantitative analysis of trimethyl-H3K9 induction by Dicer and cyclin D1 was indicated. Scale bars, 10 μm. mean ±s.e.m. representing H3K9-Tri-me staining spots in 10 randomly selected cells. **P=0.0015 for WT HCT116, and 0.0078 for Dicer−/− HCT116. The experiments were repeated independently for three times. Statistical significance was determined by the standard two-tailed Student’s t-test. (b) H3K9 trimethylation was assessed in cyclin D1+/+ versus cyclin D1−/− MEFs. Scale bars, 10 μm. (c) Western blot for trimethyl-H3K9 in control and cyclin D1 siRNA treated MCF-7 cells. (d) Western blot for Dicer in WT and Dicer−/− HCT116 cells. (e) Western blot for cyclin D1 in WT and cyclin D1−/− MEFs. ß-actin served as a loading control in all western blot analysis.
