Figure 2: LratCre efficiently labels HSCs. | Nature Communications

Figure 2: LratCre efficiently labels HSCs.

From: Fate tracing reveals hepatic stellate cells as dominant contributors to liver fibrosis independent of its aetiology

Figure 2

(a) Lrat expression was determined by western blotting in pure and never-plated primary murine HSCs (n=8), hepatocytes (n=2), Kupffer cells (n=2), endothelial cells (n=2) and cholangiocytes (n=2). (b,c) HSCs were isolated from LratCre-negative (n=2) and LratCre-positive mice (n=3) expressing ZsGreen Cre reporter and were either plated for 24 h (b) or analysed by flow cytometry (c), using vitamin A (‘VitA’, determined in the violet fluorescence-activated cell sorting channel) fluorescence as HSC marker in both approaches. (d) Co-localization of LratCre-induced ZsGreen expression and desmin was determined in untreated livers using confocal microscopy (n=4). (e) Co-localization of LratCre-induced ZsGreen expression and CD31 (marking endothelial cells), F4/80 (marking macrophages), HNF4α (marking hepatocytes) and cytokeratin (marking cholangiocytes) was determined by confocal microscopy in untreated and CCl4-treated mice. Scale bars, 100 μm (b,d,e).

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